DETERMINATION OF SOME BIOACTIVITIES OF Convallaria majalis L. (LILY OF THE VALLEY), ISOLATION PHARMACEUTICAL ACTIVE INGREDIENT AND INVESTIGATION ITS INDUSTRIAL USEAGE

In this study, some biological activities of Convallaria majalis L. (Asparagaceae), which grows naturally in Muğla and its surroundings attracts attention with pleasant smell, were determined, the isolation drug active substances industrial usability investigated. For purpose, protease enzyme that catalyzes hydrolysis proteins, is one most important groups both biochemical applications, into peptides amino acids was purified from C. majalis. Protease using TPP (Three Phase Partitioning) method. Optimum pH optimum temperature for enzyme, Km Vmax values casein, azocasein, gelatin, hemoglobin, azoalbumin substrates determined. SDS-PAGE used to check purity enzyme. The molecular weight calculated as 54.347 kDa gel filtration chromatography. effects SDS, EDTA, β-mercaptoethanol compounds Mg2+, Ca2+, Mn2+, Ni2+, Hg2+, Fe2+, Fe3+ cations, 10 mM, 1 0.1 mM concentrations on activity Volatile flavoring components analyzed Headspace GC/MSD. It determined C.majalis flowers contain volatile organic compounds, citronellol (9.6%), geraniol (8.4%), benzyalcohol (35%), phenylacetonitrile (3.0%), farnesol (1.9 %) 2,3-dihydrofarnesol (0.88 %), green grassy notes: (Z)-3-hexen-1-ol (11 (Z)-3-hexenyl acetate (7.8%). As a result work, it aromatic can be an ingredient has been concluded there use.